Lagenaria siceraria (Molina) Standl is a cucurbit which seeds are consumed by people in rural and urban Africa. This plant is subjected to a strong parasitic and diseases pressure that reduces seeds production. Efficient fight against plant parasite, particularly fungus is a prerequisite for an improved productivity. This study was undertaken in five localities (Alepe, Bondoukou, Bongouanou, Divo and Korhogo) belonging to three agroecological areas of Côte d’Ivoire. The aim was to identify fungal genera infecting L. siceraria in order to design an efficient control measure. Leaf samples with necrosis and discoloration symptoms were collected throughout the localities and subsequently, fungus were isolated and identified in laboratory. From a total of 750 samples collected, 7 types of symptoms were distinguished. Fungal genera found in all of the localities were Aspergillus, Botryosphaeria, Cochliobolus, Colletotrichum, Fusarium, Lasiodiplodia and Phoma. Only Pestalotiopsis was specific to the locality of Divo. An ANOVA test performed on the data showed a significant difference between fungal genera in terms of isolation frequency. Principal components analysis revealed that fungus distribution in each locality was correlated with climatic factors.