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Abstract
The result of laboratory analysis on bioethanol waste shows
nutritional and anti-nutritional content (HCN). Based on it, in
order to prevent environment damage then performed a
bioconversion of bioethanol waste for base material of sheep
forage by fermented previously using consortium of
Saccharomyces cerevisiae, Trichoderma viride, and
Aspergillus niger to eliminate the waste anti-nutritional content
and increase nutritional content. This research used Completely
Randomized Design (CRD) factorial pattern with two factors
and three replications. The first factor was inoculum dose (D)
and the second factor was variation of tested microorganisms
(M). The variables measured were HCN content and nutrient
content of fermented products, that were water content, protein
content and crude fiber content by proximate analysis.
Furthermore, the data was statistically analyzed by analysis of
variance (ANOVA), and was there significant difference
analyzed further by Duncan's multiple range test of 5% level.
Nutritional compositions of bioethanol waste from cassava that
had been fermented by consortium of Saccharomyces
cerevisiae, Trichoderma viride, and Aspergillus niger
experienced changes compared to before fermentation. The
results showed an increase in protein level, while fiber content,
water content, and HCN content were decrease. Bioethanol
waste from cassava fermented by Aspergillus niger and
Saccharomyces cerevisiae (k3) with 2% inoculum dose had the
highest protein content increase from 11.79% to 25.41% and
had the lowest crude fiber content decrease from 16.4% to
12.84 %. The highest HCN decrease obtained from the
fermentation of consortium Aspergillus niger and Trichoderma
viride.