The price of agricultural land in the Czech Republic is significantly lower than in other EU states; however, its fertility and method of cultivation does not differ from surrounding countries. Agricultural land area in the CR is decreasing about 12 ha/day (MoA, 2012). Arable land in the CR is losing its production value, from a food security standpoint, through the conversion of arable land into perennial cultures or permanent grassland, outplanting of fast-growing woody plants, afforestation, etc. The main aim of the research was to analyse developments in the state of agricultural land in the CR in the period 2001-2013. Result: One negative phenomenon is the fact that a larger decrease in agricultural land is happening even on higher-quality lands (an evaluation according to the average official price based on CSEU – Classified Soil-Ecological Units). The decrease in qualitatively better agricultural land in selected regions (50.1 % of the ALF CR – Agricultural Land Fund of the Czech Republic) is lower in comparison with the nationwide decrease. The development of the decrease in arable land in the three qualitatively worst regions of the CR, within the monitored period, is significantly higher than the CR average; however, farming on lower-quality lands is not economically advantageous. A deeper evaluation of twenty-one districts in the CR (30.15 % of the ALF CR) shows that only three districts are experiencing a positive development in the relative changes in the state of agricultural land in relation to the area of the district. 13 districts have relative decreases lower than the relative decrease in the CR. A partial aim was to find out whether there is any dependency between the size of a municipality (according to the number of inhabitants) and a change in the acreage of agricultural land, especially from the viewpoint of decreases in agricultural land. From a sample of 56 municipalities, no strong dependence between the monitored quantities was proven.