This study mapped and diagnosed the fresh pineapple supply chains in Benin to identify bottlenecks in pineapple quality improvement for different markets. A research framework was defined that comprised all relevant aspects to be researched. After 54 semi-structured interviews with key informants, 173 structured interviews were held with actor groups. The chain diagnosis showed there was no concordance between actor groups in which quality attribute they valued most. Moreover, pineapple quality was found to be highly heterogeneous. Key bottlenecks identified were lack of training of primary producers in production practices, unconditioned transport, and unavailability of boxes for export.