In the paper, the method of data collection, processing and visualization of the occurrence of non-indigenous and endangered animal species in the Czech Republic is described. Our new software enables easy data entry about the observation of monitored species to the expert public. The data obtained is then used by expert and scientific institutions in order to search for optimal solutions of nature protection and population management and results are open to the public. This analytic and software solution was developed by the Department of Information Technologies, Czech University of Life Sciences; the data has been also used by the Forestry and Wood Faculty and the Faculty of Life Sciences.