Availability of new herbicide-resistant biotech rice varieties may affect traditional rice rotation practices to control red rice and enhance yields. This research evaluates the adoption of biotech rice and its effects on the current practice of crop rotation in the Mississippi River Delta region. The optimal utilization of biotech rice rotation is analyzed using a nonlinear mathematical programming model with mixed integers. Optimal rotation was found to be sensitive to the technology fee, relative prices received by farmers, the effectiveness of red rice control, and a sheath blight penalty on yield for continuous rice production.