This study provides an assessment of the potential impact of trade liberalization on the regional structure of Colombian agriculture. For this, a two-step methodology is imple-mented. First, the effects of discriminatory trade liberalization are estimated by means of a multi-region general equilibrium model. Second, relevant price and quantity changes are transmitted to a simple transportation model that simulates the likely changes that trade libe-ralization will bring upon the spatial structure of agriculture. Results indicate that changes in the spatial structure of agricultural production in Colombia are not negligible and are instru-mental for policy design on several fronts.