The high agricultural process of the Argentine humid pampas forces the intensification and relocation of cattle and dairy systems into subhumid and semiarid region to keep their competitiveness. In consequence, there is an increasing water demand scenario in these fragile areas in relation with this productive transformation process. Water footprints of UHT milk and cheese agrifood chain in La Pampa and San Luis provinces have been assessed using Life Cycle Assessment (LCA) methodology, including virtual water indicators. Milk chain of La Pampa presents high self-sufficiency water ratio and high primary production proportion in virtual water exports. Water footprint of San Luis milk chain is highly externalized with a low self-sufficiency water ratio.