We outline new data on non-tariff measures (NTMs) in agricultural trade collected as part of the NTM-Impact project. The data cover product and process standards, conformity assessment measures, and country requirements for the EU and 10 other countries. We create a Heterogeneity Index of Trade (HIT) regulations to aggregate data on different measures, and estimate the impact of regulatory heterogeneity on trade using a gravity framework. Our results suggest that differences in standards reduce trade in beef and pig meat, but have little impact on trade in other agri-food products.