Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli, namely Glucoiberin (IBE); Progoitrin (PRO); Sinigrin (SIN); Glucoraphanin (RAA); Gluconapin (NAP); 4-Hydroxyglucobrassicin (4OH); Glucobrassicin (GBC); 4-Methoxyglucobrassicin (4ME); Neoglucobrassicin (NEO). Total glucosinolate content in flowers was 1-5 times higher than in leaves. The predominant glucosinolate in broccoli was glucoraphanin.