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Tracer studies with radioactive 2,4-D in bean, cotton, and cucumber plants confirmed previous findings that 2,4-D penetrates the cuticle of sprayed leaves, migrates to the phloem, and is transported in that tissue along with food materials in the plant. Freeze-dried plants were radioautographed to determine the rate and direction of translocation and the amount of chemical moved to various parts of the plant.

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