The genetic diff erentiation of the subpopulations of Ukrainian dual-purpose chickens (G-1, G-2, G-3, G-4 and C) was analyzed using microsatellite markers. It was revealed that the subpopulations of G-1 and G-4 were most remote by the values of genetics distances (28.8% of diff erences), while the G-2 and G-3 subpopulations were closest (13.3% diff erences). According to the analysis of Write's F-statistics, most of the revealed genetic variability was corresponded for by the intra-population component (9.2% of the total genetic variability was distributed between subpopulations and 90.8% within subpopulations).