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Objective: Plant life begins at germination. Stimulating germination with chemical methods can be advantageous. The pregerminative treatment of four promoters was determined in two maize genotypes (G1, G2).Design/methodology/approach: Sixty seeds per Petri dish were used as experimental unit with three repetitions, organized in a completely randomized 24 factorial design. Two factors were taken into consideration: A) Genotypes (G1, G2); and B) four pregerminative promoters. The genotypes were Antelope G1 and yellow Antelope G2. Meanwhile, the pregerminative promoters were salicylic acid (‎C7H6O3) (SA), citrulline (C6H13N3O3) (CI), humic substances derived from leonardite (HS), and tap water (TW), in 1000-ppm concentrations. The following variables were evaluated: germination percentage (GP)/days1, radicle diameter (RD), radicle length (RL), and number of lateral seminal roots (NSR). An analysis of variance and Tukey tests (0.05) were performed.Results: The germination promoters were highly significant in both genotypes, as well as during the promoter-genotype interaction. G1 and G2 means showed a higher growth and development for humic substances (HS) during germination in the NSR.Study Limitations/Implications: Germination can be inhibited, if the promoters are overweighted.Findings/Conclusions: The best genotype and germination promoter (G2) had a 94% effectiveness and HS at 1000 ppm. CI and SA registered the lowest GP.

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