Objective: To estimate the hematological and biochemical reference values in Ateles geoffroyi individuals in captivity.Design/Methodology/Approach: Eleven males and 23 females were captured and blood was collected from the coccygeal vein; conventional techniques were used to analyze the samples. Results: Mean corpuscular volume (MCV; P0.03) and platelets (PLT; P0.04) were significantly higher in females than in males. In relation to biochemical blood values, the difference was not significant in male and female groups. Study Limitations/Implications: Hematological and biochemical reference values of A. geoffroyi are within normal health parameters; they are between the ranges reported for other species of Neotropical primates and can be used as a reference in the health management of this species in captivity.Findings/Conclusions: Hematological and biochemical parameters of captive specimens of A. geoffroyi are described, which provide reference indicators for the health management of the species.