Objective. We evaluated the effect of arbuscular mycorrhizal inoculation in Citrus volkameriana Tan & Pasq plants with the application of Glomus spp. Zac- 19 (G. claroides, G. diaphanum and G. albidum) to assess growth under greenhouse conditions without fertilizer application.Methodology. The evaluated treatments were inoculation with 2,4,6,8, and 10 g of inoculum, plus a control without inoculation in an experimental design of complete randomized blocks with three replications. Variables were plant height, stem diameter, number of leaves, foliar area, dry weight, and mycorrhizal colonization.Results. A statistically significant response (p≤0.01) to inoculation was observed, registering higher growth of mycorrhizal plants. The inoculation was considered appropriate for all the levels evaluated. The outstanding treatment was 10 g of inoculum that produced plants with 110.16 cm in height for the last sampling; 38.56 leaves per plant; 1.13 cm stem diameter; 35.95 g dry weight of root; 76.88 g dry weight of the aerial part of the plant; 225.03 cm2 of leaf area per plant and 88.87% of mycorrhizal colonization.Conclusions. The application of 10 g per plant to the roots of Citrus volkameriana from the Glomus Zac-19 arbuscular mycorrhizal consortium promoted the production of more vigorous plants for grafting, without the application of chemical fertilizer.
