Objective: the in vitro mycelial growth rate of Schizophyllum commune Fr. 1815 strains from Tabasco, Mexico and the production of basidiomas in different regional agricultural substrates was evaluated. Design/methodology/approach: four strains were used: CCG003, CGG009, CCG010 and CGG13, and four substrates: cocoa peel (CP), banana leaves (BL), corn leaves (CL) and as a control, mulatto wood (MW). Based on the speed of growth, two strains and three substrates were selected to evaluate the basidiomas production in two types of containers: plastic bags and rectangular trays. Productivity was assessed based on Biological Efficiency (BE), Yield (Y) and Production Rate (PR). Results: at 12 days of incubation, the highest mycelial growth rate was recorded in CP by CCG003 and CCG013 strains (51.622.1, and 64.418.1 mm d1) and in HM and HP by CCG009 and CCG010 strains (86.917.8, and 587.8 mm d1), compared to the control substrate (from 117.27.4 to 1200.0 mm d1). High production was recorded in the CC by the CCG009 strain, grown in bags, with BE of 12.82.6%, Y of 4.10.8% and PR of 0.70.1%. The production of S. commune was better in the bag than in the tray.Limitations on study/implications: the low moisture retention of the substrate as well as the phenetic plasticity of the species significantly impact the production of basidiomas by inducing connate morphologies of a smaller size.Findings/conclusions: this study represents an advance in the production of basidiomas of this important fungus, as a food alternative for the tropical regions of Mexico.