@article{Nurtjahja:338409,
      recid = {338409},
      author = {Kiki Nurtjahja and Sartini Unk and Greaceuli Silitonga},
      title = {Fungal Contamination and Aflatoxin B1 on Postharvest  Coffee Beans in North Sumatera, Indonesia},
      journal = {International Journal of Agriculture and Environmental  Research},
      address = {2023-06-03},
      number = {2013-2023-1601},
      month = {Jun},
      year = {2023},
      abstract = {The aim of the paper is to observe aflatoxin contamination  and toxigenicity of Aspergillus flavus in relation to soil  at plantations and coffee beans during drying and storage  at smallholder plantations in Berastagi, Karo Regency,  North Sumatra. Serial dilution and a direct plating method  were used to determine the fungal population and the  percentage of beans infected by fungal species.  Toxigenicity of A. flavus was tested using a culture method  and thin-layer chromatography. The results showed a total  of 18 species of fungi were isolated from the soil  plantation. Aspergillus niger was the most dominant (log  3.69 cfu/g), followed by A. flavus (log 3.47 cfu/g) and A.  tamarii (log 3.43 cfu/g). Cladosporium cladosporioides,  Rhizopus stolonifer, and Mucor sp. contaminated the coffee  beans during drying, while Aspergillus chevalieri, A.  niger, A. repens, A. terreus, and Penicillium citrinum  contaminated the beans during storage. The highest  percentage (15%) of beans contaminated during drying was  caused by R. stolonifer and Mucor sp., whereas during  storage, A. niger (15.5%) was the dominant contaminant,  followed by A. flavus (14%). A total of eleven A. flavus  strains were isolated, consisting of three strains from the  plantation, seven strains from coffee beans during drying,  and one strain from storage. Based on toxigenicity, 10  strains of A. flavus were aflatoxin producers, with three  strains isolated from the soil, six strains isolated from  coffee beans during drying, and one strain isolated during  storage.},
      url = {http://ageconsearch.umn.edu/record/338409},
      doi = {https://doi.org/10.22004/ag.econ.338409},
}