Taking wine culture and quality of life as the research object, this paper analyzes the relationship between wine culture and quality of life. The research data were collected by questionnaire survey online, and a total of 315 valid questionnaires were collected. And then the software SPSS 21.0 is used for descriptive analysis and regression analysis. The results show that among the 12 hypotheses of the three dimensions of wine culture to the four dimensions of quality of life, 9 hypotheses are valid and 3 hypotheses are not. This study is helpful for people to clearly understand the influence and mechanism of wine culture on the quality of life.