[Objectives] To explore the effects of selenium fertilizer on rice growth and selenium enrichment. [Methods] Using high-quality rice varieties as experimental materials, exogenous selenium fertilizer was sprayed at two critical periods of tillering stage and full heading stage to explore the effects of different selenium treatments on rice agronomic traits, leaf SPAD value and yield. [Results] Spraying selenium fertilizer could promote the growth of rice, and the selenium enrichment effect in the aboveground parts of the plant was obvious. The selenium content of rice (milled rice) was 0.04-0.07 mg/kg. [Conclusions] This study lays a foundation for the promotion of suitable leaf fertilizers in Zhaoqing City, and is expected to promote the cultivation and promotion of selenium-enriched rice.