@article{XU:330030,
      recid = {330030},
      author = {XU, Jie and SUN, Li and ZHU, Wenting},
      title = {Key Technology for Virus-free Seed Potatoes Pre-elite  Breeding},
      journal = {Asian Agricultural Research},
      address = {2022-03-20},
      number = {1812-2023-067},
      month = {Mar},
      year = {2022},
      abstract = {[Objectives] To establish a breeding system for pre-elite  seed potatoes. [Methods] Shoot-tip seedlings were cultured  with different medium, and then transplanted in different  substrates. They were sprayed with nutrient solutions of  different formulas. Potato minitubers were induced.  [Results] The medium of MS+2.0 mg/L 6-BA, 1.0 mg/L NAA+3%  sugar was preferable for potato shoot tip culture. After  virus detection, the virus-free potato seedlings were  propagated. The medium composed of vermiculite, peat and  perlite (1:3:1) was recommended for transplanting potato  tissue culture seedlings in greenhouse. The  three-dimensional spray method was used to produce  pre-elite seed potatoes. The full-amount MS nutrient  solution could increase the tuber number per plant to 55.  Induction of minitubers is a better breeding technology for  pre-elite seed potatoes. The optimal medium screened for  induction of potato minitubers was MS + (5-7) mg/L 6-BA,  with a dark culture duration of about 20 d. [Conclusions]  The breeding technology established is suitable for the  two-season cultivation areas in ​​Zaozhuang.  },
      url = {http://ageconsearch.umn.edu/record/330030},
      doi = {https://doi.org/10.22004/ag.econ.330030},
}