The aim of the paper is to determine regional differentiation in the implementation of selected RDP measures 2014-2020 in the context of different natural and organizational conditions. After EU accession, Polish agricultural producers gained access to a large European market and European funds supporting agriculture and rural development. The Common Agricultural Policy (CAP), structural funds and other EU instruments have strengthened the increasingly multifaceted view of rural development. The material for analysis was data of the Agency for the Restructuring and Modernization of Agriculture (ARMA) and the Central Statistical Office (GUS). Analysis showed that the implementation of main environmental activities under the RDP 2014-2020 is, to a large extent, regionally differentiated. In relation to Less Favorited Areas, this is mainly due to natural criteria adopted for their delimitation. On the other hand, the intensity of implementation of Agri-Environmental/Agri-Environmental and Climate Measure (AE/AECM) and Organic Farming (OF) is directly correlated with average farm size. A common feature for voivodships with a larger share of AE/AECM and OF is a smaller simplification of crop rotation confirmed by a negative correlation with the share of cereal in the sown area. On this basis, it can be stated that the environmental activities of the RDP 2014-2020 implement the set goals.