The current situation on European agricultural labour markets and the expected demographic development emphasise the need to address the issues of work and working time organisation in order to attract a young generation to agriculture. The aim of this paper is to evaluate the use of modern individual methods of working time organisation within agricultural companies. Data was collected through a quantitative survey consisting of 259 active agricultural companies in the Czech Republic. Results suggest that three factors were responsible for working time organisation in agricultural companies: support of flexible employment forms, employee productivity and the use of specialists. The right working time organisation of all age groups supports the cooperation of all employees and ensures knowledge continuity.