Investment levels in agricultural businesses specializing in dairy cattle production in Polish FADN regions were compared based on data for 2004-2016. The analysis included gross and net investments (variables FADN SE516 and SE521) and investment subsidies (variable SE406). The research shows that agricultural holdings specializing in dairy cattle farming in the years 2004-2016 in the FADN Wielkopolska and Śląsk region and in the region of Pomerania and Masuria have reached the level of gross investment per one farm on average annually higher than the average annual level of gross investment in Poland. Farms specializing in dairy cattle farming in the FADN regions of Mazowsze and Podlasie, as well as Małopolska and Pogórze have achieved a lower level of gross investment per farm than on average per year in Poland. The average level of net investment per one agricultural holding specializing in dairy cattle farming in the regions of FADN Wielkopolska and Śląsk, Pomorze and Mazury as well as Mazowsze and Podlasie was of a positive value, whereas in the Małopolska and Pogórze region it was negative. Studies have shown that agricultural farms specializing in dairy cattle farming in the Wielkopolska and Śląsk regions developed the fastest among all farms in Poland, while the fastest growing farms specializing in dairy cattle farming are found in the Małopolska and Pogórze regions.