This experiment studied the biological characteristics of Colletotrichum horii causing persimmon anthracnose using the crossing method and blood cell counting plate method, and screened inhibitory fungicides via assessing the effects of 16 common fungicides on the mycelial growth and spore germination. The results showed that the most suitable temperature for mycelial growth of C. horri is 25℃, the most suitable temperature for spore germination is 28℃; the suitable pH for mycelial growth of C. horri is 4.0-6.0, the most suitable pH for spore germination is 4.0; the optimal carbon source is glucose and maltose, and the optimal source of nitrogen is beef extract. Among the 16 common fungicides, 33.5% oxine-copper suspension, bromothalonil 25% microemulsion and 70% Mancozeb wettable powder (WP) have the optimal inhibitory effects on the mycelial growth and spore germination of persimmon anthracnose pathogen, and can be used as preferred agent for prevention and control of persimmon anthracnose, followed by 70% Daisen water dispersible granules (WDG), 400 g/L flusilazole emulsion and thiram 50% WP, which can be used as alternative agents. The results are expected to provide experimental basis for effective control of persimmon anthracnose.