With seeds as experimental materials, MS was used as the basic medium to combine different species and concentrations of auxin and mitogen for proliferation, elongation and rooting culture.The best combination of medium and the most suitable medium were selected.The results showed that the best formula for the medium was MS+0.5 mg/L BA+0.5 mg/L IAA+30 g/L sucrose in the proliferation culture, MS+0.25 mg/L BA+0.5 mg/L IAA +30 g/L sucrose in the elongation culture, and MS+0.5 mg/L IAA+20 g/L sucrose in the rooting culture respectively. The experimental results will be applied in the rapid propagation and breeding of high-quality seedlings of Platycodon grandiflorus.