@article{LAI:281633,
      recid = {281633},
      author = {LAI,Xumin and WU,Peiwen and ZHENG,Siyao and ZHANG,Weijie  and PANG,Huanying and WU,Hao and XIE,Miao and HUANG,Yang  and JIAN,Jichang},
      title = {Inhibitory Effects of Prunus mume, Coptis chinensis, and  Crataegus pinnatifida on Vibrio alginolyticus and Its  Biofilm In Vitro},
      journal = {Asian Agricultural Research},
      address = {2018-08-20},
      number = {1812-2019-257},
      month = {Aug},
      year = {2018},
      abstract = {[Objectives] To explore the inhibitory effects of Prunus  mume, Coptis chinensis, and Crataegus pinnatifida on Vibrio  alginolyticus and its biofilm in vitro. [Methods] The agar  diffusion method was applied to determine the inhibition  zone diameter of three Chinese herbal medicines on V.  alginolyticus; double dilution method was used to determine  the minimum inhibitory concentration (MIC) and minimum  bactericidal concentration (MBC) of three Chinese herbal  medicines on V. alginolyticus; methylthiazolyl tetrazolium  (MTT) method was applied to evaluate the effects of three  Chinese herbal medicines on the biofilm formation of V.  alginolyticus. [Results] The three Chinese herbal medicines  had different inhibitory effects on V. alginolyticus. The  inhibition zones of P. mume, C. chinensis, and C.  pinnatifida on V. alginolyticus were (18.31 ± 0.29) mm,  (20.31 ± 0.30) mm, and (30.24 ± 0.14) mm respectively, and  P. mume had strong bacteriostatic effects on V.  alginolyticus. The double dilution method was used to  determine the bacteriostatic effects of three Chinese  herbal medicines on V. alginolyticus. Results showed that  P. mume has the highest antibacterial and bactericidal  ability. The MIC and MBC of P. mume, C. pinnatifida, and C.  chinensis on V. alginolyticus was 3.91 , 31.25 , and 125  mg/mL, respectively. Finally, MTT method was applied to  evaluate the effects of C. pinnatifida, C. chinensis, and  P. mume on the biofilm formation of V. alginolyticus, and  results showed that the MIC on the biofilm formation of V.  alginolyticus was 15.63 , 7.81,and 3.91 mg/mL,  respectively; the MIC of P. mume was the lowest.  [Conclusions] This experiment proves that P. mume, C.  chinensis, and C. pinnatifida have inhibitory effects on V.  alginolyticus and its biofilm formation. It is expected to  provide references for prevention and control of pathogenic  vibrio induced diseases of aquatic animals. },
      url = {http://ageconsearch.umn.edu/record/281633},
      doi = {https://doi.org/10.22004/ag.econ.281633},
}