The present research was conducted to assess the interaction of growth regulators and genotypes on in vitro regeneration from root tips explants of local and exotic cultivars of garlic. Root tips were cultured on MS medium supplemented with different combinations of NAA and BAP. Among those combinations 1.0 mgl-1 NNA and 4.0 mg1-1 BAP was found to be the best hormonal supplement for the highest percentage of shoot regeneration (67.33%) in Chinese genotype. Chinese genotype had higher number (5.50) of longer shoots than the local genotypes. Rooting performance was also better in Chinese genotype.