The growth performance of Spirulina platensis was studied in three different concentrations of banana leaf ash (BLA) added with 0.4 g/I jackfruit seed powder (JSP) and 0.2 g/I urea in the laboratory. S. platensis was cultured in three different concentrations viz. 4.8, 7.2 and 9.6 g/1 of BLA and Kosaric medium (KM) as control with three replications for a period of three months. Each trial was done for a period of 12 days in the laboratory. The initial cell weight of S. platensis was 4 mg/1 which attained a maximum cell weight of 545 mg/1 in Kosaric medium followed by 523, 485, and 307 mg/1 in 7.2, 4.8, and 9.6 .mg/1 of BLA media with added 0.4 g/I JSP and 0.2 g/I urea respectively, on the 8th day of culture. Similar trend was observed in case of chlorophyll a content where the highest amount was 8.12 mg/I in KM and among different concentrations of BLA highest amount was 7.53 mg/I in 7.2 g/I BLA with added 0.4 g/I JSP and 0.2 g/I urea. The percentage of crude protein, crude lipid, moisture, ash and NFE contents of JSP were recorded 12, 1.85, 10, 3.62, and 72.53%, respectively. The percentage of total nitrogen, phosphorus, sulpher, sodium and calcium of BLA were recorded 0.18, 0.09, 2.35, 0.10, 0.07 and 10.81%, respectively. The result showed that the growth rate of S. platensis was significantly (p<0.05) higher in 7.2 g/1 BLA medium with 0.4 g/I JSP and 0.2 g/I urea than other concentrations of BLA. It is suggested that BLA algal culture medium is as effective as commercially produced culture medium making it feasible to culture Spirulina.