The components of glucosinolates (GSL) in the succulent roots and leaves of three kinds of fruity radish (Xinlimei, Super Zhengyan, and Shawo) were identified and analyzed by high performance liquid chromatography (HPLC). Results showed that there were nine kinds of glucosinolates in the succulent roots and leaves of three kinds of fruity radish, including six kinds of aliphatic glucosinolates (4-methyl sulfinyl-3-butenyl glucosinolates, 2-allyl glucosinolates, 4-methyl sulfinyl butyl glucosinolates, 5-methyl sulfinyl amyl glucosinolates, 4-methyl n-butyl glucosinolates, 4-methyl thio-3- butenyl glucosinolates) and three kinds of indole glucosinolates (4-methoxy methyl indole glucosinolates, 3- methyl indole glucosinolates, 1- methoxy methyl indole glucosinolates), but there were significant differences in the content of glucosinolates between different varieties and organs. The total content of glucosinolates in succulent roots of three kinds of fruity radish was 22 472.84, 13 585.86, and 28 200.70 μg/g DM respectively, and more than 95% were aliphatic glucosinolates. The content of glucosinolates in succulent roots was 4.56, 2.71, and 4.55 times of that in leaves of three kinds of fruity radish. Main aliphatic glucosinolates in succulent roots and leaves of three kinds of fruity radish were 4-methyl sulfinyl-3-butenyl glucosinolates, accounting for 90.11%-93.92% and 63.03%-73.72% of total content of glucosinolates.