In order to provide references for popularization and application of tobacco variety Yunyan 105, a field experiment was conducted to explore the effects of different sowing and transplanting time on agronomic traits, economic benefit and quality of Yunyan 105. Results showed that the agronomic characters, quality traits and economic benefit of the tobacco were relatively fine with sowing time February 4-14 and transplanting time April 1-10. Index scores of the physical properties of tobacco leaves were greater than 80 scores with sowing time February 14 and transplanting time April 10. The comprehensive index of conventional chemical compositions was 0.39-0.73. The harmony of conventional chemical compositions of lower leaves was the best with the comprehensive index of 0.59 when the sowing time and transplanting time were February 4 and April 1 respectively. As for the middle and upper leaves the comprehensive indexes were respectively 0.71 and 0.73 with the sowing time February 14 and transplanting time April 10. The sensory evaluation of smoking quality and economic benefit were February 14 sowing and April 10 transplanting better, its taste of the tobacco leaves was pure and mild, the irritancy and the strength was less, the aftertaste was better, and the output value reached the highest 4 271.17 yuan/667 m2. The various sowing and transplanting time had no significant influence on appearance quality of tobacco leaves. The suitable sowing and transplanting time of Yunyan 105 was February 4-14 and April 1-10 respectively in Xuanwei tobacco-growing area.