@article{WANG:257311,
      recid = {257311},
      author = {WANG Mei and WU  Jiru and LIU  Yanfang and WANG Wendou},
      title = {Optimization of Extraction Conditions for SOD in Sedum  aizoon by the Response Surface Method},
      journal = {Asian Agricultural Research},
      address = {2017-01-20},
      number = {1812-2017-263},
      year = {2017},
      abstract = {This paper aimed to optimize extracting conditions of SOD  in Sedum aizoon by response surface method. First, the  single factor optimization tests were carried out. Three  single factors were time, pH and liquid-solid ratio  respectively. By Design expert 8.0.5 software, 17 kinds of  test scheme were designed. The protein yield of each scheme  was obtained, and Design expert 8.0.5 software was used to  conduct variance analysis and interactive analysis among  factors, to obtain the corresponding mathematical model.  The results showed that in single factor experiments, the  optimal time was 1 h, pH was 7.8, and liquid-solid ratio  was 15:1. The interactive analysis indicated that the  optimal scheme for extracting SOD in Sedum aizoon was time  of 0.98 h, pH of 7.79, liquid-solid ratio of 13.71:1. By  the method of pyrogallol autoxidation, the extracted SOD  enzyme activity was measured, namely 12 U/g. The results  could provide a reference for further utilization and  research of Sedum aizoon resource.},
      url = {http://ageconsearch.umn.edu/record/257311},
      doi = {https://doi.org/10.22004/ag.econ.257311},
}