@article{Davis:257055,
      recid = {257055},
      author = {Davis, M. J. and Kramer, J. B. and Ferwerda, F. H. and  Brunner, B. R.},
      title = {POSSIBLE BACTERIAL ETIOLOGY OF PAPAYA BUNCHY TOP DISEASE},
      address = {1995-07-10},
      number = {1889-2017-869},
      pages = {9},
      year = {1995},
      abstract = {Samples from 95 papaya plants with symptoms of papaya  bunchy top (PBT) were obtained with the aid of local  cooperators from 12 countries throughout the American  tropics. The samples were examined by polymerase chain  reaction (PCR) for the presence of 16S rRNA genes of  mycoplasma-like organisms, but none were found. No  differences were detectable by PCR between PBT-affected  papaya DNA samples and samples from healthy plants.  Abnormal fluorescence was consistently observed by epi  fluoresce nee microscopy in the region between the xylem  and phloem in transverse sections of PBT-affected petioles  when stained by 4',6-diamidino-2-phenylindole2HCl (DAPI) or  acridine orange. Bacteria were found in the region by  transmission electron microscopy and observed in expressed  sap by light microscopy. The bacteria were associated with  PBT-affected, but not with healthy, papaya plants. The  bacteria measured 0.25-0.35 μηι in width and 0.8-1.6 Km in  length and had a Gram-negative type cell envelope. All  attempts to isolate the bacterium in axenic culture have  failed.},
      url = {http://ageconsearch.umn.edu/record/257055},
      doi = {https://doi.org/10.22004/ag.econ.257055},
}