Sugarcane yields have declined dramatically since 1968 in Barbados, and there is an urgent need for determination of the cause. Ratoon Stunting Disease (RSD) is seen as one possible cause. In order to determine the incidence of RSD in the fields in Barbados, 3,100 plant samples were taken from the fields across the island and were tested using Evaporative Binding-Enzyme Linked Immuno Assay (EB-EIA). This survey showed the widespread occurrence of the disease in the commercial fields. Five of the commercial varieties showed more than 10% positive samples. In addition, significant differences were observed between ratoons for the five major commercial varieties. Some of the new varieties under selection also tested positive for RSD.