@article{Knights:253518,
      recid = {253518},
      author = {Knights, M. and Lambie, N. and Ramgattie, R. and  Singh-Knights, D. and Siew, N.},
      title = {EVALUATION OF TIMED ARTIFICIAL INSEMINATION PROTOCOLS IN  WATER BUFFALOES IN THE TROPICS},
      address = {2013},
      number = {537-2016-38556},
      pages = {13},
      year = {2013},
      abstract = {Three experiments were conducted to evaluate the effect of  different timed artificial insemination (TAI) protocols on  pregnancy rate (PR) in water buffaloes (.Bubalus bubalis).  In experiment 1 the efficacy of a modified ovsynch/GnRH and  progesterone-based TAI protocols was evaluated. Buffalo  cows and heifers (N = 38) assigned to Treatment 1 (n=19;  ovsynch/GnRH), received an injection of progesterone (200  mg, i.m., day-3), three (3) days later (day 0) these  animals received an injection of GnRH (Cystorelin, 100 Mg,  i.m.). Buffaloes assigned to Treatment 2 (n=19;  progesterone/CIDR-based), received a CIDR device (1.8 g of  progesterone, Pfizer, Mexico) on Day 0. On Day 7, the CIDR  device was removed and all buffaloes (N=38) were injected  with PGF2a (Lutalyse, 25 mg, i.m, 5 ml/animal i.m; Pfizer  Corporation, New York, NY). Thirty (30) hours after  injection with PGF2a all animals were injected with  estrogen (estradiol benzoate, 500 ug, i.m.), and 30 h later  (60 h post PGF2a) all animals were inseminated. Mean  pregnancy rate was numerically higher in progesterone based  (21%) than in GnRH-based (10.5%), though differences did  not attain statistical significance. Experiment 2 evaluated  the effectiveness of different ovulation synchronizing  treatments. Buffaloes (N=32) were fitted with a controlled  internal drug releasing (CIDR; 1.8g progesterone, Pfizer)  devices for seven days and injected with prostaglandin F2a  (PGF2a)· Forty eight (48) hours later (Day 9) animals were  randomly assigned to receive an injection of either  estrogen (estradiol benzoate 500 Mg, i.m.; η = 16) or GnRH  (Cystorelin 100 Mg, im; n=16). All animals were inseminated  24 h later (72 h post PGF2a)· The mean pregnancy rate  obtained was 25% and was not affected type of ovulation  synchronizing treatment or parity. Experiment 3 evaluated  the necessity of using an ovulation synchronizing agent in  TAI protocols, and the effect of time of insemination on  the PR. Water buffaloes (N=34) were treated as in  experiment 1 except 48 h after insert removal animals were  randomly assigned to receive either saline (n=21) or GnRH  (n=13). Within treatment groups buffaloes were randomly  assigned to be inseminated at 60, 72 or both 60 and 72 h  after progesterone withdrawal. The mean pregnancy rate  (41.2%) was not affected by the use of GnRH as an ovulation  synchronizing agent. The PR of animals inseminated 72 h  after CIDR removal tended to be higher (P =.10, 67%) than  animals inseminated at 60 or at both 60 and 72 h after  progesterone removal. PR also varied significantly with  inseminator. The use of CIDR-based TAI protocols with  insemination at 72 h after insert removal may be practical  tool in the management of reproduction in water buffaloes  in the tropics.},
      url = {http://ageconsearch.umn.edu/record/253518},
      doi = {https://doi.org/10.22004/ag.econ.253518},
}