This study evaluated commercial olive cultivars for resistance to olive leaf spot (OLS). A growth chamber trial was undertaken with one temperature treatment (16°C) applied during inoculation while a relative humidity (RH) of above 80% was maintained. Plants were kept for 48 hours at 99% RH after inoculation and then moved to shade netted area for disease development at a temperature of 25±5°C. The results were used for categorizing the eight olive cultivars evaluated in this study according to their OLS susceptibility. „Frantoio‟ was categorized as most tolerant; „Nandi‟ and „Leccino‟ were found to be moderately tolerant, and „Nocellara del Belice‟ fairly tolerant. „Coratina‟ was found to be the most susceptible cultivar; Barouni was found to be moderately susceptible, and „Mission‟ and „Manzanilla de Seville‟ were found to be fairly susceptible. The findings provide a basis for preliminary recommendations of OLS tolerant cultivars and selections for commercial olive production.