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Abstract
To identify tick-borne pathogens in blood samples from 68 dogs were examined for Babesia, Anaplasma/Ehrichia,
Mycoplasma, and Hepatozoon spp. by using a molecular tool. The detection of DNA was performed using specific
primers for partial 16S rRNA or 18S rRNA sequence, and amplicon sequences were analyzed. Our investigation
detected Babesia gibsoni (26 dogs) and Anaplasma sp. AnHl446 (2 dogs), Mycoplasma haemocanis (27 dogs), and
an unknown Mycoplasma spp were detected (2 dogs), which was most closely related to Candidatus Mycoplasma
turicensis, on the basis of phylogenetic analysis. Hepatozoon DNA could not be detected in this study.