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Abstract
Thirty two rice lines of BC1F1 population (Binadhan-7/FL 378) were used to identify introgressed rice lines for salt
tolerance using SSR markers at the Plant Breeding and Biotechnology Divisions of Bangladesh Institute of Nuclear
Agriculture (BINA), Mymensingh. Seeds of BC1F1 population of Binadhan-7/FL-378 were collected from backcross
population of F1 (Binadhan-7/FL 378). Salt tolerant genotype, FL-378 was crossed with high yielding variety,
Binadhan-7. Randomly selected 32 BC1F1 progenies along with their two parents (Binadhan-7, FL-378) were
genotyped with microsatellite or SSR markers for identification of introgressed rice lines. Parental polymorphism
survey was assayed by 8 SSR markers and three polymorphic SSR markers viz., RM296, RM585 and OSR30 were
selected to evaluate BC1F1 rice lines for salt tolerance. Primer RM585 indicated 16 lines as introgressed and 16 lines
as susceptible in comparison with salt tolerant parent FL-378 and salt susceptible parent Binadhan-7. Primer RM296
identified 13 introgressed, and 19 susceptible lines. Nine introgressed and 23 susceptible lines were identified when
BC1F1 lines were evaluated with marker OSR30. Line BC1F1-30, BC1F1-40, BC1F1-2, BC1F1-16 and BC1F1-34 were
identified as introgressed in comparison with parent FL-378 (salt tolerant) when RM585 and RM296 markers were
used but line BC1F1-1 and BC1F1-3 were identified as introgressed incase of 3 primers (RM585, RM296 and OSR30).
Again the lines BC1F1-5, BC1F1-24, BC1F1-26, BC1F1-27, BC1F1-29, BC1F1-31, BC1F1-37 and BC1F1-38 were found
susceptible as compared with parent Binadhan-7 when marker RM585, RM296 and OSR30 were used. The
polymorphism information content (PIC) values ranged from 0.3290 to 0.3671 with an average of 0.3544. The overall
gene diversity of SSR loci for the 32 BC1F1 rice lines along with two parents was 0.4614, ranging from 0.4152 to
0.4844. Positive correlations were found between the genetic diversity and the maximum number of repeats. Among
the lines, all the loci were polymorphic and clearly distinct and the cluster analysis (NJ tree and UPGMA) showed
nearly similar pattern of variation which could be used for improvement of salt tolerant rice lines of rice through SSR
markers.