@article{Ikram:198431,
      recid = {198431},
      author = {Ikram, Rabia and Erum, Shazia and Javed, Hina and Zaman,  Shah and Khan, Shahid and Muhammad, Aish and Kanwal, Saima},
      title = {In-Vitro Conservation of Sugarcane Germplasm},
      journal = {Asian Journal of Agriculture and Rural Development},
      address = {2014-11},
      number = {393-2016-23847},
      series = {1-508-4(11)2014-AJARD-506-509},
      pages = {4},
      year = {2014},
      abstract = {Lack of in vitro multiplication procedure has long been a  serious problem in sugarcane breeding programs. This  problem has been solved to maximum extent by In Vitro  propagation. In the present study, 6 genotypes of sugarcane  (SC 06, SC 04, SC 25, SC 11, SC 13 and SC 30) was acquired  & excised for apical and sub apical parts (eyes/ lateral  buds). The explants were surface sterilized with Clorox  (70% +100%, 20 & 10 min). Results showed that highest  survival % was achieved with apical buds (66%) as compared  to lateral buds (33%). In addition, effect of different  concentrations of  rooting media (NAA, 5% Sucrose and 6%  Sucrose) and  shooting media  (GA3, 0.1mg/, Kinetin,  0.1mg/L and BAP 0.1mg/L, Kinetin 0.1mg/L,GA3 0.1mg/L, NAA  0.1mg/L) was studied. Synergistic response was observed on  the growth of plant with hormone consortia. Significant  differences were observed in No. of roots, shoot length @  No. of shoots at all tested treatments. Highest No. of  roots recorded for SC 30 at with 6% Sucrose. Highest No. of  shoots was recorded for SC 04 with GA3 @ 0.1mg/L and  highest shoot length for SC 06 at 0.1mg/ L of Kinetin.},
      url = {http://ageconsearch.umn.edu/record/198431},
      doi = {https://doi.org/10.22004/ag.econ.198431},
}