Based on the previous research and the 1996 and 2008 land use change survey, land use type of Guangdong Province is divided into 10 types of farmland, garden plot, woodland, grassland, residential points and other construction sites, traffic and transmission land, land for water conservancy facilities, wetland, water area, and land hard to be utilized. Then, area change and annual changing rate of land use type in Guangdong Province are calculated by the analysis method of land use change. Based on this, ecosystem service value assessment method is used to discuss the variation of ecosystem service value in Guangdong Province. Result shows that ecosystem service value of land use shows a decreasing trend in Guangdong Province, having reduced from 635036 billion yuan in the year 1996 to 632394 billion yuan in the year 2008. Ecosystem service value has reduced by 2642 billion yuan, a reduction rate of only 0.4161%. Farmland, woodland, grassland, wetland, land for water conservancy facilities, and unused land have all reduced. But garden plot, traffic and transmission land, water area, and residential point and other construction site have increased. The major characteristics of land use change the years 1996-2008 in Guangdong Province are the internal adjustment of agricultural structure and the increase of construction land. Land use change and adjustment direction are basically rational and the ecological environment is stable.