Beetroot is one of horticultural crop which need intensive cultural practice. It is important throughout the world by being a source of food as well as income for consumers, processors and producers. But there is a gap of research output and information for its cultural practice especially on optimum recommended fertilizers and spacing to improve its yield in Ethiopia. A field experiment was conducted in the 2012 to study the effects of plant spacing and urea fertilization on yield and yield components of Deteroit dark red beetroot cultivars. The experiment was a randomized complete block design (RCBD) arrangement. Plant spacing (16, 20 and 25 cm within plant) were combined with three Nitrogen rates (45, 52.5 and 60 gm/plot). The general objectives that was to know the effect of spacing and urea on yield and yield component of beetroot with specific objectives that were to determine appropriate spacing and urea fertilizer rate on Beetroot for optimum yield production by keeping other agronomic practices constant, to determine appropriate interaction effect of spacing and urea for optimum yield and yield components of beetroot, and to compile information for producers, researchers and students for further improvement of the crop with regard to spacing, urea fertilizer rate and other agronomic practice.