This paper used SSR molecular markers to perform the genetic diversity analysis on 13 ordinary inbred lines of maize of different sources in order to divide heterotic groups of maize inbred line and predict heterosis. Using 12 pairs of SSR primers, a total of 47 allelic variants were detected in 13 inbred lines, 2-5 alleles were detected for each pair of primers, an average of 3.9, and polymorphism information content varied from 0.379 to 0.828. According to the cluster analysis, the 13 inbred lines could be divided into 5 groups.