The paper presents an analysis and evaluation of Agricultural Knowledge and Information Systems in Europe and the ongoing changes and trends. It is proceeded by a short description of the strategy for the bioeconomy. The analysis uses the results of the author’s own research and review of the literature. Research shows that agricultural knowledge systems are in the process of transition, i.e. from a linear top-down approach towards a more flexible bottom-up approach and interactive solutions in the form of a network. Bioeconomy represents a new challenge in front of all the classic AKIS stakeholders, as well as creates an opportunity for many new NGOs, foundations, centers of excellence, etc. specializing in various aspects of the national management of renewable resources in agriculture.