The purpose of the paper – to highlight the results of research on the formation of scientific and methodical basics for determining the effectiveness of organic land use. The theoretical and methodical basis of estimation of effectiveness of organic agricultural land use from positions of systematic and interdisciplinary approaches are revealed in the paper. The provisions on the essence and hierarchical classification of the effectiveness and economic and environmental effectiveness in particular had further development. This study presents a new system of indicators for calculating effectiveness of organic land use, beginning with an overview of different approaches to assessing agricultural land use. This system takes into account the ecosystem value (ecosystem services) and monetary valuation of agricultural lands and which includes subsystem indicators of productivity, profitability, economic stability, value and cost-effectiveness (saving). A set of key indicators for estimation of the economic effectiveness of organic agricultural land use is formed.