The strategy of 21st Century Maritime Silk Road provides favorable policy environment for deepening the economic cooperation between Guangdong and ASEAN, actively promoting the transformation and upgrading of the marine economic structure, and establishing win-win marine economic cooperative development platform. For deepening the marine cooperation with ASEAN, Guangdong has significant advantages in geographical location, humanistic foundation, marine resources and economic strength. Based on interest demands of Guangdong and ASEAN, Guangdong should build the marine economic cooperation mode and mechanism from the overall mechanism and sub-mechanism, establish a three-dimensional marine economic cooperation path with characteristics of Guangdong and ASEAN from top design, to specific cooperation level, then to implementation of supporting facilities, and expand fields of the marine economic cooperation between Guangdong and ASEAN, to promote strategic construction of 21st Century Maritime Silk Road.