Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province, this paper introduced festivals, marriage, traditional clothing, and food culture of Sani People, and present the long history and brilliant culture of Sani People. These culture resources could be used to expand the cultural aspects of leisure agriculture, to solve the problems of low participation and less distinct characteristics. This study can provide a reference for building the high-end leisure agriculture with distinct characteristics of Yunnan Province, to form a new growth point for regional growth.