The maize germplasm collection of Caribbean origin from the CIMMYT (Mexico) germplasm bank was evaluated in two stages for yield and agronomic traits in Puerto Rico. This evaluation was part of the Latin American Maize Project (LAMP), a five-stage collaboration of 12 countries in the Western Hemisphere to evaluate their native maize germplasm. The 562 accessions included in the first stage represented seven races and mixtures of these races commonly found in the Caribbean. The top 20% based on yield were re-evaluated in two locations in Puerto Rico. Yields of the top 20% ranged from 3937 to 7773 kg/ha, and 18 accessions had yield exceeding the check, a local improved variety. Accessions with good ear quality and yield were identified for use in tropical breeding programs.