MICROPROPAGATION AND FIELD PERFORMANCE OF VIRUS-FREE WHITE COCOYAM (XANTHOSOMA SAGITTIFOLIUM L. Schott) IN COSTA RICA

Apical meristem (dome with one or two leaf primordia) explants of a local virus infected white cocoyam were culture on modified Murashige and Skoog (MS) medium containing 0.1 mg. L ' 6- Bcnzylaminpurine (BA). After 120 days, complete plants were developed and above 90% of them were Dasheen Mosaic Virus (DMV) free according to ELI SA test. To induce axillary shoot formation, corm section of in vitro plants were culture on modified MS medium supplemented with 3 mg L'16- BAP. An average of 4 shoots were formed every 42 days. Shoots produced in vitro were rooted on MS without growth regulators with 100% success and after 42 days, complete plants were obtained. About 98% of the rooted in vitro plants survived transfer to the greenhouse and were successfully transplanted outdoors. Field ev aluation of two generations of in vitro virus-free white cocoyams was done. The results indicated that virus-free white cocoyams had twice the yield and better cormel quality than virus-infected plants. The establishment of a certification 'seed' program for white cocoyam is discussed.


Issue Date:
Jul 31 1994
Publication Type:
Conference Paper/ Presentation
Language:
English
Total Pages:
10




 Record created 2017-07-07, last modified 2017-08-29

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