Cucumbers are a valuable commodity throughout most of the Caribbean, including the U.S. Virgin Islands. Downy mildew, a foliar disease caused by the oomycete Pseudoperonospora cubensis (Berk. and Curt.) Rostow, is one of the most destructive pathogens of cucurbits. Cucumber growers in U.S. Virgin Islands observed disease in their crops. Nine cultivars of cucumber (slicing type) “Dasher II”, “Fanfare”, “Indio”, “Intimidator”, “Speedway”, “Stonewall”, “Thunder”, “SVR 3462”, and “SVR 4719” evaluated for disease resistance and yields in the summer of 2013 at the University of the Virgin Islands Agricultural Experiment Station. All marketable fruits were weighed to determine total yields for each cultivar. Three fruits of each cultivar were randomly selected at each harvest and measured to determine individual weight, length, and diameter. Once downy mildew infestation was significant, 50 leaves of each cultivar were randomly picked and analysed using a disease severity assessment key. “Indio” had the highest marketable yield (166.3 kg), followed by “Stonewall” (159.8 kg) and “SVR 4179” (148.7 kg). “Indio” had the significantly lowest rating of downy mildew (2.69), followed by “Speedway” (3.29) and “SVR 4179” (3.31). Matching fruit qualities to downy mildew resistance, study shows the “Indio”, “Intimidator”, and “SVR 4719” cultivars were found more suitable for U.S. Virgin Islands conditions.