To understand the spatial difference of agricultural sustainability in Tibet, this paper establishes a comprehensive evaluation model of agricultural sustainability to calculate the score of agricultural sustainability of 7 areas in 2013 in Tibet. By using cluster analysis, this paper conducts the regional evaluation of Tibet's agricultural sustainability. The results show that 7 cities' regional difference of agricultural sustainability was obvious in 2013, the agricultural sustainability index value was 0.4232-0.6937, and the value was in the order of Nyingchi> Lhasa >Shannan>Ali>Shigatse>Qamdo>Nagqu. According to the agricultural sustainable development level, Tibet can be divided into three regions: the first type is the area with the highest level of sustainable development of agriculture, including Nyingchi and Lhasa; the second type is the area with the average level of sustainable development of agriculture, including Shigatse and Shannan; the third type is the area with the low level of sustainable development of agriculture, including Qamdo, Ali and Nagqu.