Nitrogen fertilizer is an important factor for crop production. The N application strategies named as former nitrogen moved backward (FNMB) are tested in three ecological regions to optimize the N application in rice. The dry matter accumulation and distribution, yield and quality are studied to understand the formation of yield and quality of rice under different N application strategies. The result indicates that former nitrogen moved backward (FNMB) can increase tiller number and dry matter accumulation; effective ears and yield can be increased with the increase of fertilization; rational nitrogen application can help to establish scientific group structure, harmonize yield component, and then achieve high ratio of input to output and benefit.